Chromatography Virtual Lab Simulation PDF Worksheet

This engaging virtual chromatography lab allows students to model the process of separating mixtures and identifying substances using Rf values—without needing any lab equipment. Perfect for classrooms with limited lab access, remote learning, flipped lessons, or review of practical skills.

Students begin by placing dye samples onto chromatography paper, run the solvent front, and watch as the dyes separate based on solubility and polarity. They then use a movable on-screen ruler to collect measurements, record results in a structured data table, and calculate Rf values. Finally, students compare their results to reference data to identify unknown dyes.

This resource is ideal for MYP Science, IGCSE and general middle and high school chemistry/biology units that introduce chromatography or separation techniques.

What’s Included

✔ Structured Student Worksheet (PDF) including:

• Clear step-by-step instructions

• Data table for measurements and Rf calculations

• Analysis and conclusion questions

• Identification task using known dye Rf values

Skills Developed

• Accurate measurement and data recording

• Calculation of Rf values

• Interpretation of chromatographic results

• Using reference data to identify unknown substances

• Scientific reasoning and evidence-based explanations

Curriculum Connections

• MYP Sciences: Strand-specific inquiry, data analysis, and evaluation

• IGCSE / GCSE: Separation techniques and chromatography (Chemistry and Combined Science)

Why Teachers Love This Resource

✅ Easy to use — just open and go
✅ Encourages hands-on thinking even without lab access
✅ Builds genuine practical and analytical skills
✅ Compatible with Chromebooks, iPads, laptops & whiteboards
✅ Perfect for class, homework, revision, or blended learning